| Product Name | Mouse anti-JAM-1, clone M.Ab.F11 (Monoclonal) |
|---|---|
| Description | Mouse anti-JAM-1, clone M.Ab.F11 (Monoclonal) |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | M.Ab.F11 |
| Immunogen | Unknown or proprietery to MONOSAN and/or its suppliers |
| Isotype | IgG1 |
| Reactivity | Human |
| Applications | ELISA, FA, FC, IF, IHC-P, IP, WB |
| Working Dilutions | ,,,,,, |
| Diluent Buffer | PBS with 0.1% BSA and 0.02% sodium azide |
| Concentration | 100 ug/ ml |
| Storage | 2-8°C |
| Notes |
The monoclonal antibody M.Ab.F11 recognizes junctional adhesion molecule-A (JAM-A) also known as the human platelet F11-Receptor (F11R) or JAM-1. JAM-A is a surface glycoprotein duplex (32 and 35 kDa) belongingto the immunoglobulin superfamily found on the surface of human platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-A belongs together with JAM-C (JAM-2) and JAM-B (VE-JAM or JAM-3) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM-A plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interactions. It co-distributes with other tight junction components such as ZO-1, 7H6 antigen, cingulin and occludin. Moreover, JAM-A plays a role in platelet aggregation, secretion, adhesion, spreading. In the platelet, JAM-A is a membrane protein involved in 2 distinct processes initiated on the platelet surface. Namely,, antibody-induced platelet aggregation and secretion both dependent on FcgammaRII and GPIIb/IIIa integrin, a process that may be involved in pathophysiological processes associated with certain thrombocytopenias and secondly, antibody mediated platelet adhesion independent from FcgammaRII or- fibrinogen receptor that appears to play a role in physiological processes associated with platelet adhesion and aggregation. A physiological role for the JAM-A protein was demonstrated by its phosphorylation after the stimulation of platelets by thrombin and collagen. A pathophysiological role for the JAM-A was revealed by demonstrating the presence of JAM-A antibodies in patients with thrombocytopenia. Adhesion of platelets through JAM-A resulted in events characteristic of the action of cell adhesion molecules. Recent data suggests a role for JAM-A in the adhesion of platelets to cytokine-inflamed endothelial cells and thus in thrombosis and atherosclerosis induced in non-denuded blood vessels by inflammatory processes. |
| References | Kornecki; E et al. J Biol Chem 1990; 265: 10042. Naik, U et al Biochem J 1995, 310: 155. Wang; F et al. Biochem J 1995; 311: 401. Sobocka M et al. Blood 2000; 95: 2600. Babinska A et al. Thromb Haemost 2002; 87: 712 |
| Supplier | MONOSAN |
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