| Product Name | Mouse anti-PAI-1, clone MA-33H1F7 (Monoclonal) |
|---|---|
| Description | Mouse anti-PAI-1, clone MA-33H1F7 (Monoclonal) |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MA-33H1F7 |
| Immunogen | Unknown or proprietery to MONOSAN and/or its suppliers |
| Isotype | IgG1 |
| Reactivity | Human, Mouse, Rabbit, Rat |
| Applications | ELISA, FA, WB |
| Working Dilutions | ,, |
| Diluent Buffer | PBS with 0.1% BSA and 0.02% sodium azide |
| Concentration | 100 ug/ ml |
| Storage | 2-8°C |
| Notes |
Plasminogen activator inhibitor type-1 (PAI-1), a member of the serine protease inhibitor (serpin) superfamily, is an important protein in the regulation of fibrinolysis. PAI-1 is unique among the serpins because of its functional and conformational flexibility. PAI-1 is the most important physiological inhibitor of both tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u- PA). Increased PAI-1 levels are associated with thrombotic events and is an established risk factor for cardiovascular diseases. The active conformation PAI-1 inhibits its target proteinases by the formation of a stable, inactive complex. Although PAI-1 is synthesized as an active molecule, it converts spontaneously to an inactive, latent form that can be partially reactivated by denaturing agents. In addition, a third conformation reacting as a non-inhibitory substrate towards various target proteinases has been identified. The epitope of monoclonal antibody MA-33H1F7 is predominantly composed of three residues (Lys154/Glu130/Arg131), positioned virtually linearly in the three-dimensional structure. The epitope of the antibody does not cover the complete alpha-helix F and turn connecting alpha-helix F and beta-strand s3A, but is restricted to the hinge region between alpha-helix F and the main part of the PAI-1 molecule. The monoclonal antibody MA-33H1F7 is a ‘switching’ antibody, capable of inducing a non-inhibitory substrate form of PAI-1. It was shown to inhibit PAI-1 in a dose dependent manner. |
| References | Debrock; S et al. Biochim Biophys Acta 1997; 1337: 257. Berry, C et al Br J Pharm 1998, 125: 29. Bijnens; A et al. J Biol Chem 2000; 275: 6375. Rupin A et al. Thromb Haemst 2001; 86: 1528. Sironi L et al. J Am Heart Ass 2001; 37: 961 |
| Supplier | MONOSAN |
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