Mouse Bone Morphogenetic Protein 1 ELISA Kit

Category: Antibodies
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A2375
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Product Name Mouse Bone Morphogenetic Protein 1 ELISA Kit
Synonyms H3R26me2a, HIST1H3J, H3/j , H3FJ , Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h , Histone H3/I, Histone H3/j , Histone H3/k, Histone H3/l,
Host Rabbit
Method Sandwich
Detection Range 78.125 - 5000 pg/ml
Sensitivity 30 pg/ml
Immunogen A synthetic methylated peptide corresponding to residues surrounding Arg26 of human histone H3
Isotype IgG
Molecular Weight 15kDa
Target Bone Morphogenetic Protein 1
Reactivity Mouse
Applications ELISA
Working Dilutions WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:200
Sample Types Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Form Liquid, in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Reconstitution The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Purity Affinity purification
Gene Id 8356
Uniprot P68431
Storage Store at -20C. Avoid freeze/thaw cycles.
Supplier Antibodies.com

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