Mouse GDP-fucose protein O-fucosyltransferase 2 (POFUT2) ELISA Kit
| Product Name | Mouse GDP-fucose protein O-fucosyltransferase 2 (POFUT2) ELISA Kit |
|---|---|
| Description | Mouse GDP-fucose protein O-fucosyltransferase 2 (POFUT2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse POFUT2. No significant cross-reactivity or interference between Mouse POFUT2 and analogues was observed. POFUT2 is an enzyme responsible for adding fucose sugars in O linkage to serine or threonine residues in Thrombospondin repeats. The protein is an inverting glycosyltransferase, which means that the enzyme uses GDP-β-L-fucose as a donor substrate and transfers the fucose in O linkage to the protein producing fucose-α-O-serine/threonine. Almost all glycosyltransferases reside in the Golgi apparatus. However, POFUT2 as well as the related enzyme POFUT1 have recently been shown to reside in the endoplasmic reticulum.The deduced protein contains 428 amino acids. RT-PCR ELISA detected moderate expression in all adult and fetal tissues and specific brain regions examined. Crystal structures of POFUT2 reveal a variation of the classical GT-B fold and identify sugar donor and TSR acceptor binding sites. This Mouse GDP-fucose protein O-fucosyltransferase 2 (POFUT2) ELISA Kit employs a two-site sandwich ELISA to quantitate POFUT2 in samples. An antibody specific for POFUT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOFUT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POFUT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POFUT2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | POFUT2, C21orf80, FUT13, |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Mouse |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 80294 |
| Storage | 2-8°C |
| Background |
POFUT2 is an enzyme responsible for adding fucose sugars in O linkage to serine or threonine residues in Thrombospondin repeats. The protein is an inverting glycosyltransferase, which means that the enzyme uses GDP-β-L-fucose as a donor substrate and transfers the fucose in O linkage to the protein producing fucose-α-O-serine/threonine. Almost all glycosyltransferases reside in the Golgi apparatus. However, POFUT2 as well as the related enzyme POFUT1 have recently been shown to reside in the endoplasmic reticulum.The deduced protein contains 428 amino acids. RT-PCR ELISA detected moderate expression in all adult and fetal tissues and specific brain regions examined. Crystal structures of POFUT2 reveal a variation of the classical GT-B fold and identify sugar donor and TSR acceptor binding sites. |
| Supplier | Abbkine |
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