| Product Name | Mouse Protein diaphanous homolog 1 (DIAPH1) ELISA Kit |
|---|---|
| Description | Mouse Protein diaphanous homolog 1 (DIAPH1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DIAPH1. No significant cross-reactivity or interference between Mouse DIAPH1 and analogues was observed. Diaph1 encodes a member of the formin family of proteins that play important roles in cytoskeletal rearragnement by nucleation of actin filaments. Mice lacking the encoded protein develop age-dependent myeloproliferative defects resembling human myeloproliferative syndrome and myelodysplastic syndromes. Trafficking of T lymphocytes to secondary lymphoid organs and egression of thymocytes from the thymus are impaired in these animals. Lack of the encoded protein in T lymphocytes and thymocytes also reduces chemotaxis. Alternative splicing results in multiple transcript variants encoding different isoforms. This Mouse Protein diaphanous homolog 1 (DIAPH1) ELISA Kit employs a two-site sandwich ELISA to quantitate DIAPH1 in samples. An antibody specific for DIAPH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIAPH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIAPH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIAPH1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | DIAPH1, DFNA1, DIA1, DRF1, FLJ25265, LFHL1, hDIA1, diaphanous 1, diaphanous-related formin 1 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Mouse |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 13367 |
| Storage | 2-8°C |
| Background | Diaph1 encodes a member of the formin family of proteins that play important roles in cytoskeletal rearragnement by nucleation of actin filaments. Mice lacking the encoded protein develop age-dependent myeloproliferative defects resembling human myeloproliferative syndrome and myelodysplastic syndromes. Trafficking of T lymphocytes to secondary lymphoid organs and egression of thymocytes from the thymus are impaired in these animals. Lack of the encoded protein in T lymphocytes and thymocytes also reduces chemotaxis. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Supplier | Abbkine |
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