| Product Name | MRPL48, 29-212aa, Human (Denatured) |
|---|---|
| Description | Mammalian mitochondrial ribosomal proteins help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 6p. Recombinant human MRPL48 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | Mitochondrial ribosomal protein L48, CGI-118, HSPC290, L48MT, MRP-L48 |
| Host | E. coli |
| Molecular Weight | 34.9 kDa (329aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSSGEKPIY SVGGILLSIS RPYKTKPTHG IGKYKHLIKA EEPKKKKGKV EVRAINLGTD YEYGVLNIHL TAYDMTLAES YAQYVHNLCN SLSIKVEESY AMPTKTIEVL QLQDQGSKML LDSVLTTHER VVQISGLSAT FAEIFLEIIQ SSLPEGVRLS VKEHTEEDFK GRFKARPELE ELLAKLK |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17 Lai C.-H., et al. (2000) Genome Res. 10:703-713 |
| Background | Mammalian mitochondrial ribosomal proteins help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 6p. Recombinant human MRPL48 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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