| Product Name | MRPS2, 1-296aa, Human |
|---|---|
| Description | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. MRPS2 is a 28S subunit protein that belongs to the ribosomal protein S2 family. Recombinant human MRPS2 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | Mitochondrial ribosomal protein S2, CGI-91, MRP-S2, S2mt |
| Host | E. coli |
| Molecular Weight | 14.5 kDa (133aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMATSSAA LPRILGAGAR APSRWLGFLG KATPRPARPS RRTLGSATAL MIRESEDSTD FNDKILNEPL KHSDFFNVKE LFSVRSLFDA RVHLGHKAGC RHRFMEPYIF GSRLDHDIID LEQTATHLQL ALNFTAHMAY RKGIILFISR NRQFSYLIEN MARDCGEYAH TRYFRGGMLT NARLLFGPTV RLPDLIIFLH TLNNIFEPHV AVRDAAKMNI PTVGIVDTNC NPCLITYPVP GNDDSPLAVH LYCRLFQTAI TRAKEKRQQV EALYRLQGQK EPGDQGPAHP PGADMSHSL |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Suzuki T., et al (2001). J. Biol. Chem. 276:33181-33195 Koc E.C., et al (2001). J. Biol. Chem. 276:19363-19374 |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. MRPS2 is a 28S subunit protein that belongs to the ribosomal protein S2 family. Recombinant human MRPS2 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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