| Product Name | MRPS23, 1-190aa, Human (Denatured) |
|---|---|
| Description | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS23 encodes a 28S subunit protein. Recombinant human MRPS23 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | 28S ribosomal protein S23 mitochondrial, 28S ribosomal protein S23, mitochondrial, CGI-138, HSPC329, MRP-S23 |
| Host | E. coli |
| Molecular Weight | 24.0 kDa(213aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMAGSRLE TVGSIFSRTR DLVRAGVLKE KPLWFDVYDA FPPLREPVFQ RPRVRYGKAK APIQDIWYHE DRIRAKFYSV YGSGQRAFDL FNPNFKSTCQ RFVEKYTELQ KLGETDEEKL FVETGKALLA EGVILRRVGE ARTQHGGSHV SRKSEHLSVR PQTALEENET QKEVPQDQHL EAPADQSKGL LPP |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH7.4) containing 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Lai C.-H., et al (2000). Genome Res. 10:703-713 Kenmochi N., et al (2001). Genomics 77:65-70 |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS23 encodes a 28S subunit protein. Recombinant human MRPS23 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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