| Product Name | MRPS28, 72-187aa, Human |
|---|---|
| Description | Mammalian mitochondrial ribosomal proteins help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature. Recombinant human MRPS28 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | 28S ribosomal protein S28 mitochondrial, 28S ribosomal protein S28, mitochondrial, HSPC007, MRP-S28, MRP-S35, MRPS35 |
| Host | E. coli |
| Molecular Weight |
25.5 kDa (236aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSGSPKNVE SFASMLRHSP LTQMGPAKDK LVIGRIFHIV ENDLYIDFGG KFHCVCRRPE VDGEKYQKGT RVRLRLLDLE LTSRFLGATT DTTVLEANAV LLGIQESKDS RSKEEHHEK |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Phosphate buffer saline (pH 8.0) containing 30% glycerol, 1mM DTT |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Zhang Q.-H., et al. (2000) Genome Res. 10:1546-1560. Kenmochi N., et al. (2001) Genomics 77:65-70 |
| Background | Mammalian mitochondrial ribosomal proteins help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature. Recombinant human MRPS28 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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