| Product Name | MSH6 Monoclonal Antibody |
|---|---|
| Description | MutS protein homolog 6 (MSH6) is part of the mismatch repair (MMR) pathway which is utilized by normal proliferating cells to repair mutations that may occur during DNA replication. Antibodies to MSH6 are a useful aid for classification of tumors of the gastrointestinal tract, including associated extracolonic cancers.Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated Lys-36 of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction. |
| Synonyms | DNA mismatch repair protein Msh6 ,hMSH6,G/T mismatch-binding protein,GTBP,GTMBP,MutS-alpha 160 kDa subunit,p160 |
| Host | Mouse |
| Clone | PT0169 |
| Immunogen | Full length protein from eukaryotic expression system. |
| Isotype | IgG |
| Specificity | This antibody detects endogenous levels of MSH6 |
| Reactivity | Human |
| Applications | IHC |
| Form | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Uniprot | P52701 |
| Background | MutS protein homolog 6 (MSH6) is part of the mismatch repair (MMR) pathway which is utilized by normal proliferating cells to repair mutations that may occur during DNA replication. Antibodies to MSH6 are a useful aid for classification of tumors of the gastrointestinal tract, including associated extracolonic cancers.Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated Lys-36 of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction. |
| Supplier | Elabscience |
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