| Product Name | MSRA, 24-235aa, Human |
|---|---|
| Description | MSRA (methionine sulfoxide reductase A ) belongs to the MsrA Met sulfoxide reductase family. This enzyme has an important function as a repair enzyme for proteins that have been inactivated by oxidation. It catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. In enzymology, a MSRA is an enzyme that catalyzes the chemical reaction. The 3 substrates of this enzyme are peptide-L-methionine, thioredoxin disulfide, and H2O, whereas its two products are peptide-L-methionine (R)-S-oxide and thioredoxin. Recombinant human MSRA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | methionine sulfoxide reductase A, bA235O14.2, NRK1, RP11-235O14.2 |
| Host | E. coli |
| Molecular Weight | 42.4 kDa (399aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMGNSAS NIVSPQEALP GRKEQTPVAA KHHVNGNRTV EPFPEGTQMA VFGMGCFWGA ERKFWVLKGV YSTQVGFAGG YTSNPTYKEV CSEKTGHAEV VRVVYQPEHM SFEELLKVFW ENHDPTQGMR QGNDHGTQYR SAIYPTSAKQ MEAALSSKEN YQKVLSEHGF GPITTDIREG QTFYYAEDYH QQYLSKNPNG YCGLGGTGVS CPVGIKK |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.15M NaCl, 1mM DTT |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Boschi-Muller S, et al. (2005) Biochim. Biophys. Acta. 1703 (2): 231-8. Hansel A., et al. (2005) Biochim. Biophys. Acta 1703:239-247. |
| Background | MSRA (methionine sulfoxide reductase A ) belongs to the MsrA Met sulfoxide reductase family. This enzyme has an important function as a repair enzyme for proteins that have been inactivated by oxidation. It catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. In enzymology, a MSRA is an enzyme that catalyzes the chemical reaction. The 3 substrates of this enzyme are peptide-L-methionine, thioredoxin disulfide, and H2O, whereas its two products are peptide-L-methionine (R)-S-oxide and thioredoxin. Recombinant human MSRA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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