| Product Name | Myl9, 1-172aa, Mouse |
|---|---|
| Description | Myl9 also known as myosin regulatory light polypeptide 9 is one of the numerous regulatory myosin light chains. Regulatory myosin light chains regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of regulatory myosin light chains is catalyzed by MLCK in the presence of calcium and calmodulin and it increases the actin-activated myosin ATPase activity, thereby regulates the contractile activity. Recombinant mouse Myl9 was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Myosin regulatory light polypeptide 9, AI327049, MLC20, Mylc2c, RLC-C |
| Host | E. coli |
| Molecular Weight | 47.1kDa (441aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMSSKRA KAKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DEYLEGMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEASG FIHEDHLREL LTTMGDRFTD EEVDEMYREA PIDKKGNFNY VEFTRILKHG AKDKDD |
| Tag | His-tag |
| Reactivity | Mouse |
| Applications | SDS-PAGE |
| Form | Liquid, Liquid, in Phosphate buffered saline (pH7.4) containing 10% glycerol, 1mM DTT |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Ma Z., et al. (2006) Mol. Cell. Biol. 26:9016-9034 |
| Background | Myl9 also known as myosin regulatory light polypeptide 9 is one of the numerous regulatory myosin light chains. Regulatory myosin light chains regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of regulatory myosin light chains is catalyzed by MLCK in the presence of calcium and calmodulin and it increases the actin-activated myosin ATPase activity, thereby regulates the contractile activity. Recombinant mouse Myl9 was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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