| Product Name | NAPSA, 64-420aa, Human (Denatured) |
|---|---|
| Description | NAPSA belongs to the peptidase A1 family and may be involved in processing of pneumocyte surfactant precursors. The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. Recombinant human NAPSA protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | Aspartyl protease 4, Aspartyl protease 4, Napsin-A, KAP, Kdap, NAP1, NAPA, SNAPA, ASP4 |
| Host | E. coli |
| Molecular Weight | 48.1kDa (439aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSKPIFVPL SNYRDVQYFG EIGLGTPPQN FTVAFDTGSS NLWVPSRRCH FFSVPCWLHH RFDPKASSSF QANGTKFAIQ YGTGRVDGIL SEDKLTIGGI KGASVIFGEA LWEPSLVFAF AHFDGILGLG FPILSVEGVR PPMDVLVEQG LLDKPVFSFY LNRDPEEPDG GELVLGGSDP AHYIPPLTFV PVTVPAYWQI HMERVKVGPG LTLCAKGCAA ILDTGTSLIT GPTEEIRALH AAIGGIPLLA GEYIILCSEI PKLPAVSFLL GGVWFNLTAH DYVIQTTRNG VRLCLSGFQA LDVPPPAGPF WILGDVFLGT YVAVFDRGDM KSSARVGLAR ARTRGADLGW GETAQAQFPG |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in PBS buffer (pH 7.4) containing 10% glycerol |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Aulakh KS., et al. (2013) Arch. Pathol. Lab. Med. 137 (8), 1094-1098 Chernock RD, et al. (2013) Am. J. Surg. Pathol. 37 (8), 1215-1222 |
| Background | NAPSA belongs to the peptidase A1 family and may be involved in processing of pneumocyte surfactant precursors. The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. Recombinant human NAPSA protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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