| Product Name | NCS1, 1- 190aa, Human, Recombinant |
|---|---|
| Description | Neuronal calcium sensor 1, also known as NCS1, is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. NCS1 is localized to neuronal cell bodies and axons throughout the brain and spinal cord. It is also expressed in glial cells and in neuroendocrine bovine adrenal chromaffin and PC12 cells. NCS1 is a regulatory protein involved in Ca++-dependent exocytosis of synaptic vesicles and dense core granules. Recombinant human NCS1 fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Frequenin homolog, FLUP, FREQ, Neuronal calcium sensor 1 Flup, Freq, frequenin (Drosophila) homolog, Frequenin-like protein, NCS1, Neuronal Calcium Sensor 1. |
| Host | E.coli |
| Molecular Weight | 20.9 kDa (187aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV LEHHHHHH |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT. |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Cox J A., et al. (1994) J Biol Chem. 269:32807-32813. McFerran., et al. (1998) J Biol Chem. 273:22768-22772. |
| Supplier | ARP |
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