| Product Name | NDUFS4, 43-175aa, Human, Recombinant |
|---|---|
| Description | NDUFS4, also known as NADH dehydrogenase (ubiquinone) Fe-S protein 4, is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. Recombinant human NDUFS4 protein was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | NADH dehydrogenase (ubiquinone) Fe-S protein 4, AQDQ, NADH dehydrogenase (ubiquinone) Fe-S protein 4 CI 18 kDa, CI AQDQ, Complex I 18 kDa, Complex I AQDQ, mitochondrial respiratory chain complex I (18 KD subunit), NADH dehydrogenase, NADH ubiquinone oxidoreductase 18 kDa subunit. |
| Host | E. coli |
| Molecular Weight | 51.2 kDa (460aa) (Molecular weight on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MAQDQTQDTQ LITVDEKLDI TTLTGVPEEH IKTRKVRIFV PARNNMQSGV NNTKKWKMEF DTRERWENPL MGWASTADPL SNMVLTFSTK EDAVSFAEKN GWSYDIEERK VPKPKSKSYG ANFSWNKRTR VSTK |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM MES buffer (pH 6.0) containing 2mM EDTA, 0.1mM PMSF, 10% glycerol |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Choi WS., et al. (2008), Proc Natl Acad Sci U S A. 105(39):15136-41. De Rasmo D., et al. (2008). Cell Signal. 20(5):989-97. |
| Background | NDUFS4, also known as NADH dehydrogenase (ubiquinone) Fe-S protein 4, is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. Recombinant human NDUFS4 protein was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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