| Product Name | NFu1, 10-254aa, Human |
|---|---|
| Description | NFu1 is a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. This protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of bmultiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human NFu1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | NFu1 iron-sulfur cluster scaffold homolog mitochondrial, NFu1 iron-sulfur cluster scaffold homolog, mitochondrial, CGI-33, HIRIP, HIRIP5, MMDS1, Nfu, Nifu, NIFuC |
| Host | E. coli |
| Molecular Weight | 23.6 kDa (213aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSGAAAVAA GLRRRFCHML KNPYTIKKQP LHQFVQRPLF PLPAAFYHPV RYMFIQTQDT PNPNSLKFIP GKPVLETRTM DFPTPAAAFR SPLARQLFRI EGVKSVFFGP DFITVTKENE ELDWNLLKPD IYATIMDFFA SGLPLVTEET PSGEAGSEED DEVVAMIKEL LDTRIRPTVQ EDGGDVIYKG FEDGIVQLKL QGSCTSCPSS IITLKNGIQN MLQFYIPEVE GVEQVMDDES DEKEANSP |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tirs-HCl (pH8.0) containing 10% glycerol |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Purity | >90 % by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Ganesh S., et al (2003). Hum. Mol. Genet. 12:2359-2368 Tong W.-H., et al (2003). Proc. Natl. Acad. Sci. u.S.A. 100:9762-9767 |
| Background | NFu1 is a protein that is localized to mitochondria and plays a critical role in iron-sulfur cluster biogenesis. This protein assembles and transfers 4Fe-4S clusters to target apoproteins including succinate dehydrogenase and lipoic acid synthase. Mutations in this gene are a cause of bmultiple mitochondrial dysfunctions syndrome-1, and pseudogenes of this gene are located on the short arms of chromosomes 1 and 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human NFu1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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