NGAL ELISA kit (Human)

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SEB388Hu
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Product Name NGAL ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Neutrophil Gelatinase Associated Lipocalin (NGAL). No significant cross-reactivity or interference between Neutrophil Gelatinase Associated Lipocalin (NGAL) and analogues was observed.
Synonyms Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2
Method Sandwich
Detection Range 0.156-10ng/mL
Sensitivity 0.065ng/mL
Target NGAL
Specificity This assay has high sensitivity and excellent specificity for detection of Neutrophil gelatinase-associated lipocalin (NGAL). No significant cross-reactivity or interference between Neutrophil gelatinase-associated lipocalin (NGAL) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma, Urine, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot P80188
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Correlation of serum neutrophil gelatinase-associated lipocalin with acute kidney injury in hypertensive disorders of pregnancy;Cholesterol Contributes to Diabetic Nephropathy through SCAP-SREBP-2 Pathway;Lipocalin-2: Response To Resistance Training in Obese Young Men;Effects of Resistance versus Endurance Training on Plasma Lipocalin-2 in Young Men;Differences in plasma and sputum biomarkers between COPD and COPD-asthma overlap;NGAL ;Hs-CRP and Adipokin (Lcn2): Response to Exercise Training in Obese Men;A Study On Thyroid Hormone And Its Effects On Exercise ;、118 ;Correlation of serum neutrophil gelatinase associated lipocalin with disease severity in hypertensive disorders of pregnancy;Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease;Increased cardiac index attenuates septic acute kidney injury: a prospective observational study;;From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.;Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol;Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis.;Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN);From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases;Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery;Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypes;Potential Novel Biomarkers of Obstructive Nephropathy in Children with Hydronephrosis;The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy;The evaluation of acute kidney injury due to ischemia by urinary neutrophil gelatinase-induced lipocalin (uNGAL) measurement in patients who underwent partial …;CОСТОЯНИЕ ТИРЕОИДНОЙ ФУНКЦИИ У ПАЦИЕНТОВ С САХАРНЫМ ДИАБЕТОМ 1-ГО ТИПА И ХРОНИЧЕСКОЙ БОЛЕЗНЬЮ ПОЧЕК …;Thyroid function in patients with type 1 diabetes mellitus and chronic kidney disease receiving renal replacement therapy;Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers;Elevated plasma level of neutrophil gelatinase-associated lipocalin (NGAL) in patients with breast cancer;
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