| Product Name | NNMT, 1-264aa, Human (Bioactivity Validated) |
|---|---|
| Description | NNMT(Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Nicotinamide N-methyltransferase |
| Host | E. coli |
| Molecular Weight |
47.9kDa (434aa) 40-57kDa (SDS-PAGE under reducing conditions) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE GLFSLVARKL SRPL |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. |
| Concentration | 0.5mg/ml (determined by Absorbance at 280nm) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Parsons RB., et al. (2002). J Neuropathol Exp Neurol. 61(2):111-24. Smith ML., et al. (1998). Biochim Biophys Acta. 1442(2-3):238-44. |
| Background | NNMT(Nicotinamide N-methyltransferase) belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. It is predominantly expressed in the liver, and a lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. NNMT catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Recombinant human NNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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