| Product Name | NQO1, 1-274aa, Human, Recombinant |
|---|---|
| Description | NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This protein apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinines thus preventing the formation of reactive oxygen species. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). Recombinant NQO1 protein was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | DHQU, DIA4, DTD, NMOR1, NMORI, QR1, Azoreductase, Quinone reductase 1, DT-diaphorase, NAD(P)H dehydrogenase, quinone 1, Azoreductase.Menadione reductase, NQO 1, QR 1, Phylloquinone reductase, Cytochrome b 5 reductase, DIA 4, Diaphorase (NADH/NADPH), Diaphorase 4 Dioxin inducible 1, DT diaphorase, Diaphorase (NADH/NADPH) (cytochrome b 5 reductase), NAD(P)H dehydrogenase quinone 1 NAD(P)H menadione oxidoreductase 1 dioxin inducible, NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1, NAD(P)H:menadione oxidoreductase 1, NAD(P)H:Quinone acceptor oxidoreductase type 1, NAD(P)H:quinone oxidoreductase 1, NAD(P)H:quinone oxireductase, NMOR 1, NMOR I. |
| Host | E. coli |
| Molecular Weight | 14.3 kDa (126aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 10mM Sodium Citrate pH 3.5 containing 10% Glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Jaiswal AK., et al. (1988) J Biol Chem. 263(27):13572-8. Traver RD., et al. (1992) Cancer Res. 52(4):797-802. |
| Background | NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This protein apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. NQO1 functions as an important part of cellular antioxidant defense by detoxifying quinines thus preventing the formation of reactive oxygen species. Altered expression of NQO1 has been seen in many tumors and is also associated with Alzheimer's disease (AD). Recombinant NQO1 protein was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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