| Product Name | NuBP1, 1-320aa, Human |
|---|---|
| Description | NuBP1 also known as cytosolic Fe-S cluster assembly factor NuBP1 isoform 1. NuBP1 is implicated in the regulation of centrosome duplication by similarity. This protein is component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. It required for maturation of extra mitochondrial Fe-S proteins The NuBP1-NuBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of a Fe-S cluster and its transfer to target apoproteins. Recombinant human NuBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Cytosolic Fe-S cluster assembly factor NuBP1 isoform 1, Cytosolic Fe-S cluster assembly factor NuBP1 isoform 1, NBP, NBP1, NBP35 |
| Host | E. coli |
| Molecular Weight | 79.4kDa (709aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMEEVPHD CPGADSAQAG RGASCQGCPN QRLCASGAGA TPDTAIEEIK EKMKTVKHKI LVLSGKGGVG KSTFSAHLAH GLAEDENTQI ALLDIDICGP SIPKIMGLEG EQVHQSGSGW SPVYVEDNLG VMSVGFLLSS PDDAVIWRGP KKNGMIKQFL RDVDWGEVDY LIVDTPPGTS DEHLSVVRYL ATAHIDGAVI ITTPQEVSLQ DVRKEINFCR KVKLPIIGVV ENMSGFICPK CKKESQIFPP TTGGAELMCQ DLEVPLLGRV PLDPLIGKNC DKGQSFFIDA PDSPATLAYR SIIQRIQEFC NLHQSKEENL ISS |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, Liquid, in Phosphate buffered saline (pH7.4) containing 10% glycerol, 1mM DTT |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Stehling O., et al. (2008) Mol. Cell. Biol. 28:5517-5528. Bienvenut W.V., et al. (2012) Mol. Cell. Proteomics 11:M111.015131-M111.015131 |
| Background | NuBP1 also known as cytosolic Fe-S cluster assembly factor NuBP1 isoform 1. NuBP1 is implicated in the regulation of centrosome duplication by similarity. This protein is component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. It required for maturation of extra mitochondrial Fe-S proteins The NuBP1-NuBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of a Fe-S cluster and its transfer to target apoproteins. Recombinant human NuBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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