| Product Name | PBK, 1-322aa, Human |
|---|---|
| Description | Lymphokine-activated killer T-cell-originated protein kinase, also known as PBK, is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase (MAPKK) family. PBK is abundant in placenta and absent from adult brain tissue. A PDZ domain in the tumor suppressor protein Dlg can coordinate with the T/SXV motif of PBK. This protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Recombinant human PBK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Lymphokine-activated killer T-cell-originated protein kinase, CT84, Nori-3, SPK, TOPK |
| Host | E. coli |
| Molecular Weight | 42 kDa (395aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMEGISN FKTPSKLSEK KKSVLCSTPT INIPASPFMQ KLGFGTGVNV YLMKRSPRGL SHSPWAVKKI NPICNDHYRS VYQKRLMDEA KILKSLHHPN IVGYRAFTEA NDGSLCLAME YGGEKSLNDL IEERYKASQD PFPAAIILKV ALNMARGLKY LHQEKKLLHG DIKSSNVVIK GDFETIKICD VGVSLPLDEN MTVTDPEACY IGTEPWKPKE AVEENGVITD KADIFAFGLT LWEMMTLSIP HINLSNDDDD EDKTFDESDF DDEAYYAALG TRPPINMEEL DESYQKVIEL FSVCTNEDPK DRPSAAHIVE ALETDV |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Abe Y., et al. (2000) J Biol Chem. 275:21525-21531. Dougherty J D., et al. (2005) J Neurosci. 25:10773-10785. |
| Background | Lymphokine-activated killer T-cell-originated protein kinase, also known as PBK, is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase (MAPKK) family. PBK is abundant in placenta and absent from adult brain tissue. A PDZ domain in the tumor suppressor protein Dlg can coordinate with the T/SXV motif of PBK. This protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Recombinant human PBK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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