| Product Name | PEDF, 20-418aa, Human, Recombinant |
|---|---|
| Description | PEDF, also known as SERPINF1, is an extracellular neurotrophic agent, first identified as a secreted product in conditioned medium from cultured fetal human retinal pigment epithelial (RPE) cells. It was reported that PEDF isolated from medium conditioned by human fetal RPE primary cultures promotes neurite outgrowth in cultured human retinoblastoma Y-79 cells. PEDF is also a member of the serpin family, a group of serine protease inhibitors. As PEDF does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Recombinant PEDF protein containing a natural variant M72T was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Pigment epithelium-derived factor, EPC-1, PIG35, SERPINF1, Pigment epithelium-derived factor EPC 1, EPC1, PIG 35, Pigment epithelium derived factor, Proliferation inducing protein 35, SERPIN F1, Serpin peptidase inhibitor clade F member 1, SERPINF 1, |
| Host | E. coli |
| Molecular Weight | 64.6 kDa (570aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYDLYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ PAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol. |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Steele FR., et al. (1993) Proc Natl Acad Sci U S A. 90(4):1526-30.. Becerra SP., et al. (1995) J Biol Chem. 270(43):25992-9. |
| Background | PEDF, also known as SERPINF1, is an extracellular neurotrophic agent, first identified as a secreted product in conditioned medium from cultured fetal human retinal pigment epithelial (RPE) cells. It was reported that PEDF isolated from medium conditioned by human fetal RPE primary cultures promotes neurite outgrowth in cultured human retinoblastoma Y-79 cells. PEDF is also a member of the serpin family, a group of serine protease inhibitors. As PEDF does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Recombinant PEDF protein containing a natural variant M72T was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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