Product Name | PFDN2, 1-154aa, Human |
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Description | PFDN2 (Prefoldin subunit 2) is a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. This protein binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. And binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Recombinant human PFDN2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Synonyms | Prefoldin subunit 2, PFD2, HSPC231 |
Host | E.coli |
Molecular Weight | 23.7 kDa (214aa) confirmed by MALDI-TOF |
Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MAENSGRAGK SSGSGAGKGA VSAEQVIAGF NRLRQEQRGL ASKAAELEME LNEHSLVIDT LKEVDETRKC YRMVGGVLVE RTVKEVLPAL ENNKEQIQKI IETLTQQLQA KGKELNEFRE KHNIRLMGED EKPAAKENSE GAGAKASSAG VLVS |
Tag | His-tag |
Reactivity | Human |
Applications | SDS-PAGE |
Form | Liquid, in 20 mM Tris-HCl Buffer (pH 7.5) containing 10% Glycerol |
Concentration | 1mg/ml (determined by Bradford assay) |
Purity | > 90% by SDS-PAGE |
Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
References |
Vainberg I.E., et al. (1998) Cell 93:863-873 Grebenova D., et al. (2006) Blood Cells Mol. Dis. 37(3):210-7. |
Supplier | ARP |
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