PHF5A, 1-110aa, Human

Proteins, Recombinant
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Product Name PHF5A, 1-110aa, Human
Applications SDS-PAGE
Background PHF5A is a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. PHF5A contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. Recombinant human PHF5A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Concentration 0.5 mg/ml (determined by Bradford assay)
Form Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Species Human
Host E.coli
Intended Use For Research Use Only
Molecular Weight 13.2kDa (120aa)
NCBI Accession # NP_116147
Purity > 85% by SDS-PAGE
References Wagner SA, et al. (2011) Mol Cell Proteomics, PMID 21890473.
Storage Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Synonyms PHD finger-like domain-containing protein 5A, PHD finger protein 5A, bK223H9.2, INI, Rds3, SAP14b, SF3b14b
Tag His-tag

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