| Product Name | Pig Acyl-CoA desaturase (SCD) ELISA Kit |
|---|---|
| Description | Pig Acyl-CoA desaturase (SCD) ELISA Kit has high sensitivity and excellent specificity for detection of Pig SCD. No significant cross-reactivity or interference between Pig SCD and analogues was observed. Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an iron-containing enzyme that catalyzes a rate-limiting step in the synthesis of unsaturated fatty acids. The principal product of SCD is oleic acid, which is formed by desaturation of stearic acid. The ratio of stearic acid to oleic acid has been implicated in the regulation of cell growth and differentiation through effects on cell membrane fluidity and signal transduction. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 and SCD5 (MIM 608370), have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates . This Pig Acyl-CoA desaturase (SCD) ELISA Kit employs a two-site sandwich ELISA to quantitate SCD in samples. An antibody specific for SCD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySCD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCD bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | SCD, PRO1933, FADS5, MSTP008, SCD1, SCDOS, acyl-CoA desaturase, delta-9-desaturase, fatty acid desaturase, predicted protein of HQ0998, stearoyl-CoA desaturase 1, stearoyl-CoA desaturase opposite strand |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Porcine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Supplier | Abbkine |
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