| Product Name | PLA2G7, 47-441aa, Human |
|---|---|
| Description | PLA2G7(platelet-activating factor acetylhydrolase), also known as lipoprotein-associated phospholipase A2, is a phospholipase A2 enzyme. It is a secreted enzyme which catalyzes the degradation of platelet-activating factor to biologically inactive products. This protein is produced by inflammatory cells and hydrolyzes oxidised phospholipids in LDL. In the blood, PLA2G7 goes mainly with LDL and less than 20% is coupled with HDL. It is implicated in the development of atherosclerosis and is also a marker for cardiac disease. It might have a major physiologic effect in the presence of inflammatory bodily responses. Recombinant human PLA2G7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Platelet-activating factor acetylhydrolase precursor, Platelet-activating factor acetylhydrolase precursor, LDL-PLA2, LP-PLA2, PAFAD, PAFAH |
| Host | E. coli |
| Molecular Weight | 23.8kDa (210aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSAAASFGQ TKIPRGNGPY SVGCTDLMFD HTNKGTFLRL YYPSQDNDRL DTLWIPNKEY FWGLSKFLGT HWLMGNILRL LFGSMTTPAN WNSPLRPGEK YPLVVFSHGL GAFRTLYSAI GIDLASHGFI VAAVEHRDRS ASATYYFKDQ SAAEIGDKSW LYLRTLKQEE ETHIRNEQVR QRAKECSQAL SLILDIDHGK PVKNALDLKF DMEQLKDSID REKIAVIGHS FGGATVIQTL SEDQRFRCGI ALDAWMFPLG DEVYSRIPQP LFFINSEYFQ YPANIIKMKK CYSPDKERKM ITIRGSVHQN FADFTFATGK IIGHMLKLKG DIDSNVAIDL SNKASLAFLQ KHLGLHKDFD QWDCLIEGDD ENLIPGTNIN TTNQHIMLQN SSGIEKYN |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Phosphate buffer containing 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Tjoelker LW. et al. (1995) Nature. 374: 549-553 |
| Background | PLA2G7(platelet-activating factor acetylhydrolase), also known as lipoprotein-associated phospholipase A2, is a phospholipase A2 enzyme. It is a secreted enzyme which catalyzes the degradation of platelet-activating factor to biologically inactive products. This protein is produced by inflammatory cells and hydrolyzes oxidised phospholipids in LDL. In the blood, PLA2G7 goes mainly with LDL and less than 20% is coupled with HDL. It is implicated in the development of atherosclerosis and is also a marker for cardiac disease. It might have a major physiologic effect in the presence of inflammatory bodily responses. Recombinant human PLA2G7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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