| Product Name | PLIN2, 1-437aa, Human, T7 tag |
|---|---|
| Description | PLIN2 belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Recombinant human PLIN2, fused to T7-tag at N-terminus, was expressed in E.coli. |
| Synonyms | adipose differentiation-related protein, ADFP, ADRP, MGC10598 |
| Host | E. coli |
| Molecular Weight | 53.0kDa (470aa) |
| Amino Acid Sequence | MASMTGGQQM GRGSMASVAV DPQPSVVTRV VNLPLVSSTY DLMSSAYLST KDQYPYLKSV CEMAENGVKT ITSVAMTSAL PIIQKLEPQI AVANTYACKG LDRIEERLPI LNQPSTQIVA NAKGAVTGAK DAVTTTVTGA KDSVASTITG VMDKTKGAVT GSVEKTKSVV SGSINTVLGS RMMQLVSSGV ENALTKSELL VEQYLPLTEE ELEKEAKKVE GFDLVQKPSY YVRLGSLSTK LHSRAYQQAL SRVKEAKQKS QQTISQLHST VHLIEFARKN VYSANQKIQD AQDKLYLSWV EWKRSIGYDD TDESHCAEHI ESRTLAIARN LTQQLQTTCH TLLSNIQGVP QNIQDQAKHM GVMAGDIYSV FRNAASFKEV SDSLLTSSKG QLQKMKESLD DVMDYLVDNT PLNWLVGPFY PQLTESQNAQ DQGAEMDKSS QETQRSEHKT H |
| Tag | T7 tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | >85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Shaw,C.S.,, et al. (2012) Am. J. Physiol. Endocrinol. Metab. 303 (9), E1158-E1165 Peters,S.J., et al. (2012) Appl Physiol Nutr Metab 37 (4), 724-735 |
| Background | PLIN2 belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Recombinant human PLIN2, fused to T7-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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