| Product Name | POLR2J, 1-117aa, Human |
|---|---|
| Description | POLR2J is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This protein exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. Recombinant human POLR2J protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | DNA-directed RNA polymerase II subunit RPB11-a, hRPB14, POLR2J1, RPB11, RPB11A, RPB11m |
| Host | E. coli |
| Molecular Weight | 41.1 kDa (359aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Proshkin,S.A., et al. (2011) Biochemistry Mosc. 76 (8), 976-980 Shpakovskii,D.G., et al. (2004) Bioorg. Khim. 30 (6), 621-625 |
| Background | POLR2J is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This protein exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. Recombinant human POLR2J protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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