| Product Name | PPIL4, 1-492aa, Human |
|---|---|
| Description | PPIL4, also known as HDCME13P, is evolutionarily conserved member of the cyclophilin-type PPIase family of proteins. Ubiquitously expressed with predominant expression in kidney, PPIL4 localizes to the nucleus and contains one PPIase cyclophilin-type domain, a lysine-rich domain, a pair of bipartite nuclear targeting sequences and one RRM (RNA recognition motif) domain. The presence of the RRM domain along with nuclear targeting sequences suggests that PPIL4 may be involved in transcriptional regulation. Recombinant human PPIL4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Peptidyl-prolyl cis-trans isomerase-like 4, HDCME13P, Rotamase PPIL4, Cyclophilin-like protein PPIL4 |
| Host | E. coli |
| Molecular Weight | 34.2 kDa (309aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MAVLLETTLG DVVIDLYTEE RPRACLNFLK LCKIKYYNYC LIHNVQRDFI IQTGDPTGTG RGGESIFGQL YGDQASFFEA EKVPRIKHKK KGTVSMVNNG SDQHGSQFLI TTGENLDYLD GVHTVFGEVT EGMDIIKKIN ETFVDKDFVP YQDIRINHTV ILDDPFDDPP DLLIPDRSPE PTREQLDSGR IGADEEIDDF KGRSAEEVEE IKAEKEAKTQ AILLEMVGDL PDADIKPPEN VLFVCKLNPV TTDEDLEIIF SRFGPIRSCE VIRDWKTGES LCYAFIEFEK EEDCEKAFFK MDNVLIDDRR IHVDFSQSVA KVKWKGKGGK YTKSDFKEYE KEQDKPPNLV LKDKVKPKQD TKYDLILDEQ AEDSKSSHSH TSKKHKKKTH HCSEEKEDED YMPIKNTNQD IYREMGFGHY EEEESCWEKQ KSEKRDRTQN RSRSRSRERD GHYSNSHKSK YQTDLYERER SKKRDRSRSP KKSKDKEKSK YR |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 85 % by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Nebert DW., et al. (2004) Hum Genomics. 1(5):381-8. Valle C., et al. (2005) Parasitol Res. 96(4):199-205. |
| Background | PPIL4, also known as HDCME13P, is evolutionarily conserved member of the cyclophilin-type PPIase family of proteins. Ubiquitously expressed with predominant expression in kidney, PPIL4 localizes to the nucleus and contains one PPIase cyclophilin-type domain, a lysine-rich domain, a pair of bipartite nuclear targeting sequences and one RRM (RNA recognition motif) domain. The presence of the RRM domain along with nuclear targeting sequences suggests that PPIL4 may be involved in transcriptional regulation. Recombinant human PPIL4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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