| Product Name | PPP1R2, 1-166aa, Human |
|---|---|
| Description | PPP1R2 is an inhibitor of protein-phosphatase 1 and forms heterodimer with PP1. Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex. Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex. Recombinant human PPP1R2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Protein phosphatase 1, regulatory (inhibitor) subunit 2, IPP-2; IPP2 |
| Host | E. coli |
| Molecular Weight | 16.9 kDa (148aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMAASTAS HRPIKGILKN KTSTTSSMVA SAEQPRGNVD EELSKKSQKW DEMNILATYH PADKDYGLMK IDEPSTPYHS MMGDDEDACS DTEATEAMAP DILARKLAAA EGLEPKYRIQ EQESSGEEDS DLSPEEREKK RQFEMKRKLH YNEGLNIKLA RQLISKDLH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 1mM DTT |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Sanseau P., Jackson A, et al. (1994), Genome 5:490-496 |
| Background | PPP1R2 is an inhibitor of protein-phosphatase 1 and forms heterodimer with PP1. Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex. Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex. Recombinant human PPP1R2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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