| Product Name | PRDX4, 38-271aa, Human |
|---|---|
| Description | PRDX4 is an antioxidant enzyme and belongs to the peroxiredoxin (Prdx, Prx, or TrxPx) family. This protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein regulates the of the transcription factor NF-kappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. Recombinant human PRDX4, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques. |
| Synonyms | PRX-4, AOE37-2, PRDX4, peroxiredoxin 4 Antioxidant enzyme 372, Antioxidant enzyme AOE372, AOE37 2, AOE372, EC 1.11.1.15, Peroxiredoxin4, PRDX 4, PRX 4, Prx IV, PRX4, PrxIV, Thioredoxin dependent peroxide reductase A0372, Thioredoxin peroxidase, Thioredoxin Peroxidase (Antioxidant Enzyme), Thioredoxin peroxidase AO372, TRANK. |
| Host | E. coli |
| Molecular Weight | 25.9 kDa (230aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH7.5) containing 100mM NaCl, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Jin D.-Y., et al. (1997) J. Biol. Chem. 272:30952-30961. Wang HQ., et al. (2009) FEBS Lett. 583(9):1511-5. |
| Background | PRDX4 is an antioxidant enzyme and belongs to the peroxiredoxin (Prdx, Prx, or TrxPx) family. This protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein regulates the of the transcription factor NF-kappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. Recombinant human PRDX4, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques. |
| Supplier | ARP |
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