| Product Name | PRKAA, 1, 1-279aa, Human |
|---|---|
| Description | PRKAA1 also known as 5-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1. PRKAA1 is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinasen activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Recombinant human PRKAA1 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | 5-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1, AMPK, AMPKa11 |
| Host | E. coli |
| Molecular Weight | 56.9kDa (516aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMRRLSSW RKMATAEKQK HDGRVKIGHY ILGDTLGVGT FGKVKVGKHE LTGHKVAVKI LNRQKIRSLD VVGKIRREIQ NLKLFRHPHI IKLYQVISTP SDIFMVMEYV SGGELFDYIC KNGRLDEKES RRLFQQILSG VDYCHRHMVV HRDLKPENVL LDAHMNAKIA DFGLSNMMSD GEFLRTSCGS PNYAAPEVIS GRLYAGPEVD IWSSGVILYA LLCGTLPFDD DHVPTLFKKI CDGIFYTPQY LNPSVISLLK HMLQVDPMKR ATIKDIREHE WF |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, Liquid, in Phosphate buffered saline (pH7.4) containing 10% glycerol, 1mM DTT |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | >85 % by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Imamura K., et al. (2001) Biochem. Biophys. Res. Commun. 287:562-567 Yang W., et al. (2001) J. Biol. Chem. 276:38341-38344 |
| Background | PRKAA1 also known as 5-AMP-activated protein kinase catalytic subunit alpha-1 isoform 1. PRKAA1 is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinasen activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Recombinant human PRKAA1 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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