Human 11,12-DHETs(11,12-dihydroxyeicosatrienoic acids) ELISA Kit

Catalog
E-EL-H2358
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Assay Kits, ELISA, Human ELISA, Elabscience
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Product Name Human 11,12-DHETs(11,12-dihydroxyeicosatrienoic acids) ELISA Kit
Species Human
Description This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human 11,12-DHETs. During the reaction, Human 11,12-DHETs in the sample or standard competes with a fixed amount of Human 11,12-DHETs on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human 11,12-DHETs. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human 11,12-DHETs in the samples is then determined by comparing the OD of the samples to the standard curve.
Synonyms 11, 12-dihydroxyeicosatrienoic acids
Method Competitive ELISA
Detection Range 1.563-100ng/mL
Sensitivity 0.938ng/mL
Sample Types Serum, Plasma, Cell supernatant
FDA Status For research use only, not for use in diagnostic procedures.
Antigen Name 11,12-DHETs

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