| Product Name | Protein Kinase C alpha (PKC-a), human |
|---|---|
| Description | Recombinant human PKC-alpha (rHuPKC-a). rHuPKC-a is a non-glycosylated polypeptide containing amino acids 2-672 having a molecular mass of 80kDa. This protein is the full-length form of the protein with an amino acid terminal poly His-tag. |
| Synonyms | C-terminal hemopexin-like domain of MMP-2, PEX |
| Molecular Weight | 76.7 KD |
| Amino Acid Sequence | MADVYPGNDS TASQDVANRF ARKGALRQKN VHEVKDHKFI ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPDTDDPRS KHKFKIHTYG SPTFCDHCGS LLYGLIHQGM KCDTCDMNVH KQCVINVPSL CGMDHTEKRG RIYLKAEVAD EKLHVTVRDA KNLIPMDPNG LSDPYVKLKL IPDPKNESKQ KTKTIRSTLN PQWNESFTFK LKPSDKDRRL SVEIWDWDRT TRNDFMGSLS FGVSELMKMP ASGWYKLLNQ EEGEYYNVPI PEGDEEGNME LRQKFEKAKL GPAGNKVISP SEDRKQPSNN LDRVKLTDFN FLMVLGKGSF GKVMLADRKG TEELYAIKIL KKDVVIQDDD VECTMVEKRV LALLDKPPFL TQLHSCFQTV DRLYFVMEYV NGGDLMYHIQ QVGKFKEPQA VFYAAEISIG LFFLHKRGII YRDLKLDNVM LDSEGHIKIA DFGMCKEHMM DGVTTRTFCG TPDYIAPEII AYQPYGKSVD WWAYGVLLYE MLAGQPPFDG EDEDELFQSI MEHNVSYPKS LSKEAVSICK GLMTKHPAKR LGCGPEGERD VREHAFFRGI DWEKLENREI QPPFKPKVCG KGAENFDKFF TRGQPVLTPP DQLVIANIDQ SDFEGFSYVN PQFVHPILQS AV |
| Biological Activity | 1570 nmole of phosphate transferred to Histone H1 substrate per minute per mg of total protein at 30C. Activity determined at a final protein concentration of 0.5 to 2?g/ml |
| Form | Purified, in 20mM HEPES pH 7.5, 250mM NaCl, 50% glycerol, 2mM EDTA,2mM EGTA, 0.05%Triton X-100 and 5mM DTT. |
| Purity | > 95%, as determined by SDS-PAGE |
| Storage | 2-8C for immediate use, or at -2°C (aliquot) |
| Supplier | ARP |
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