| Product Name | Protein Quantification Kit (BCA Assay) |
|---|---|
| Description | Abbkine Protein Quantification Kit (BCA Assay) provides a simple, rapid, detergent-compatible procedure for the detection and quantitation of total protein. |
| Synonyms | Bicinchoninic acid protein |
| Detection Range | 20-2,000 µg/mL |
| Sensitivity | 20 µg/mL |
| Reconstitution | The BCA method is suitable for the detection of protein concentrations in the range of 20-2,000 µg/mL, the minimum detected protein amount is 0.4 µg, and the sample volume to be tested is 1-20 µL. |
| Storage | Store at room temperature, stable for 12 months |
| Notes |
• 1. Use a microplate reader to measure the absorbance at 562 nm, or the absorbance at other wavelengths between 540-595 nm. • 2. The protein concentration determined by BCA method is not affected by chemical substances in most samples, and can be compatible with up to 5% SDS, 5% Triton X-100, 5% Tween20, 60, 80 in the sample. However, the BCA method is affected by chelating agents and slightly higher concentrations of reducing agents. It is necessary to ensure that EDTA is less than 10 mM, no EGTA, dithiothreitol (DTT) is less than 1 mM, and β-mercaptoethanol (β-Mercaptoethanol) is less than 0.01%. • 3. It is recommended to make a standard curve for each measurement. Because the color of the BCA method will continue to deepen with the extension of time, and the color reaction will be accelerated due to the increase of temperature. • 4. If the sample diluent or lysate itself has a high background, please try the Protein Quantification Kit (Bradford Assay) (Cat #:KTD3002). |
| Supplier | Abbkine |
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