| Product Name | Protein Quantification Kit (Bradford Assay) |
|---|---|
| Description | Abbkine Protein Quantification Kit (Bradford Assay) provides a simple and rapid procedure for determining the concentration of protein in samples. |
| Synonyms | Coomassie |
| Detection Range | 50-1000 µg/ml |
| Sensitivity | 25 µg/ml |
| Reconstitution |
Abbkine Protein Quantification Kit (Bradford Assay) provides a simple and rapid procedure for determining the concentration of protein in samples. The method utilizes an improved Coomassie blue G reagent which forms a blue complex in the presence of protein. When coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465nm to 595nm with a concomitant color change from brown to blue. The intensity of the blue complex is proportional to the amount of protein in the sample. |
| Storage | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Notes | Due to the influence of high concentration detergent, it is necessary to ensure that the concentration of SDS is lower than 0.1 %, Triton X-100 is lower than 0.1 % and Tween 20, 60, 80 is lower than 0.06 % in the sample. |
| Supplier | Abbkine |
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