| Product Name | PSAT1, 1-370aa, Human |
|---|---|
| Description | PSAT1, also known as phosphoserine aminotransferase, catalyses the conversion of 3-phosphohydroxypyruvate into 3-phosphoserine which is dephosphorylated subsequently by phosphoserine phosphatase to form L-serine. Recombinant human PSAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Phosphoserine aminotransferase, EPIP, PSA, PSAT |
| Host | E. coli |
| Molecular Weight | 19.8 kDa (182aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMDAPRQ VVNFGPGPAK LPHSVLLEIQ KELLDYKGVG ISVLEMSHRS SDFAKIINNT ENLVRELLAV PDNYKVIFLQ GGGCGQFSAV PLNLIGLKAG RCADYVVTGA WSAKAAEEAK KFGTINIVHP KLGSYTKIPD PSTWNLNPDA SYVYYCANET VHGVEFDFIP DVKGAVLVCD MSSNFLSKPV DVSKFGVIFA GAQKNVGSAG VTVVIVRDDL LGFALRECPS VLEYKVQAGN SSLYNTPPCF SIYVMGLVLE WIKNNGGAAA MEKLSSIKSQ TIYEIIDNSQ GFYVCPVEPQ NRSKMNIPFR IGNAKGDDAL EKRFLDKALE LNMLSLKGHR SVGGIRASLY NAVTIEDVQK LAAFMKKFLE MHQL |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 50% glycerol, 0.3M NaCl, 5mM DTT, 2mM EDTA |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Choudhary C., et al. (2009) Science. 325:834-840 Hart C.E., et al. (2007) Am. J. Hum. Genet. 80:931-937 |
| Background | PSAT1, also known as phosphoserine aminotransferase, catalyses the conversion of 3-phosphohydroxypyruvate into 3-phosphoserine which is dephosphorylated subsequently by phosphoserine phosphatase to form L-serine. Recombinant human PSAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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