| Product Name | PSMB6, 35-239aa , Human |
|---|---|
| Description | PSMB6 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by PSMB9. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Recombinant human PSMB6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Proteasome subunit beta type-6 precursor, DELTA, LMPY, MGC5169 |
| Host | E. coli |
| Molecular Weight | 41 kDa (358aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MTTIMAVQFD GGVVLGADSR TTTGSYIANR VTDKLTPIHD RIFCCRSGSA ADTQAVADAV TYQLGFHSIE LNEPPLVHTA ASLFKEMCYR YREDLMAGII IAGWDPQEGG QVYSVPMGGM MVRQSFAIGG SGSSYIYGYV DATYREGMTK EECLQFTANA LALAMERDGS SGGVIRLAAI AESGVERQVL LGDQIPKFAV ATLPPA |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl. |
| Concentration | 1 mg/ml (determined by Bradford) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Apcher GS., et al. (2003) FEBS Lett. 553(1-2):200-4. Stelzl U., et al. (2002) Cell. 122(6):957-68. |
| Background | PSMB6 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by PSMB9. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Recombinant human PSMB6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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