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|Product Name||PSMG4, 1-123aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MEGLVVAAGG DVSLHNFSAR LWEQLVHFHV MRLTDSLFLW VGATPHLRNL AVAMCSRYDS IPVSTSLLGD TSDTTSTGLA QRLARKTNKQ VFVSYNLQNT DSNFALLVEN RIKEEMEAFP EKF|
|Background||Proteasome assembly chaperone 4, also known as PSMG4, is a chaperone protein which promotes assembly of the 20S proteasome. It interacts with PSMG3, as well as directly alpha and beta subunits of the 20S proteasome but dissociates before the formation of half-proteasomes, probably upon recruitment of POMP. Recombinant human PSMG4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||0.5mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 40% glycerol, 200mM NaCl|
|Intended Use||For Research Use Only|
|Molecular Weight||31.7kDa (274aa), confirmed by MALDI-TOF|
|NCBI Accession #||NP_001122063|
|Purity||> 95% by SDS-PAGE|
|References||Le Tallec B., et al. (1998) Mol Cell. 17(4):660-74.
Ramos PC., et al. (1998) Cell. 20(4): 489-99.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||Proteasome assembly chaperone 4, PAC4|
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