| Product Name | PYCR2, 1-320aa, Human |
|---|---|
| Description | PYCR2 also known as pyrroline-5-carboxylate reductase 2 isoform 1, belongs to the pyrroline-5-carboxylate reductase family. This protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. The 3 substrates of this enzyme are L-proline, NAD+, and NADP+, whereas its 4 products are 1-pyrroline-5-carboxylate, NADH, NADPH, and H+. Recombinant human PYCR2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Pyrroline-5-carboxylate reductase 2 isoform 1, P5CR2 |
| Host | E. coli |
| Molecular Weight |
57.2kDa (514aa), Reducing conditions 50-70KDa (SDS-PAGE under reducing conditions.) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMSVGFIG AGQLAYALAR GFTAAGILSA HKIIASSPEM NLPTVSALRK MGVNLTRSNK ETVKHSDVLF LAVKPHIIPF ILDEIGADVQ ARHIVVSCAA GVTISSVEKK LMAFQPAPKV IRCMTNTPVV VQEGATVYAT GTHALVEDGQ LLEQLMSSVG FCTEVEEDLI DAVTGLSGSG PAYAFMALDA LADGGVKMGL PRRLAIQLGA QALLGAAKML LDSEQHPCQL KDNVCSPGGA TIHALHFLES GGFRSLLINA VEASCIRTRE LQSMADQEKI SPAALKKTLL DRVKLESPTV STLTPSSPGK LLTRSLALGG KKD |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in phosphate buffered saline (pH 7.4) containing 10% glycerol. |
| Concentration | 0.25mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | De Ingeniis J, et al. (2012). PLoS One. 7(9):e45190. |
| Background | PYCR2 also known as pyrroline-5-carboxylate reductase 2 isoform 1, belongs to the pyrroline-5-carboxylate reductase family. This protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. The 3 substrates of this enzyme are L-proline, NAD+, and NADP+, whereas its 4 products are 1-pyrroline-5-carboxylate, NADH, NADPH, and H+. Recombinant human PYCR2, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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