| Product Name | QPCT, 29-361aa, Human, Baculovirus |
|---|---|
| Description | QPCT, also known as glutaminyl-peptide cyclotransferase, is responsible for the biosynthesis of pyroglutamyl peptides. This protein has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. It catalyzes N-terminal pyroglutamate formation. In vitro, it catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-beta-amyloid. Recombinant human QPCT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Synonyms | Glutaminyl-peptide cyclotransferase, GCT, QC, sQC |
| Host | Virus |
| Molecular Weight |
29.7kDa (267aa) 28-40KDa (SDS-PAGE under reducing conditions.) |
| Amino Acid Sequence | VSPSASAWPE EKNYHQPAIL NSSALRQIAE GTSISEMWQN DLQPLLIERY PGSPGSYAAR QHIMQRIQRL QADWVLEIDT FLSQTPYGYR SFSNIISTLN PTAKRHLVLA CHYDSKYFSH WNNRVFVGAT DSAVPCAMML ELARALDKKL LSLKTVSDSK PDLSLQLIFF DGEEAFLHWS PQDSLYGSRH LAAKMASTPH PPGARGTSQL HGMDLLVLLD LIGAPNPTFP NFFPNSARWF ERLQAIEHEL HELGLLKDHS LEGRYFQNYS YGGVIQDDHI PFLRRGVPVL HLIPSPFPEV WHTMDDNEEN LDESTIDNLN KILQVFVLEY LHLHHHHHH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. |
| Concentration | 0.5mg/ml (determined by Absorbance at 280nm) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Schilling S. et al., (2004) FEBS Lett. 563:191-196. Huang K.F. et al., (2011) J. Biol. Chem. 286:12439-12449. |
| Background | QPCT, also known as glutaminyl-peptide cyclotransferase, is responsible for the biosynthesis of pyroglutamyl peptides. This protein has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue. It catalyzes N-terminal pyroglutamate formation. In vitro, it catalyzes pyroglutamate formation of N-terminally truncated form of APP amyloid-beta peptides [Glu-3]-beta-amyloid. Recombinant human QPCT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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