| Product Name | RAB3D, 1-219aa, Human |
|---|---|
| Description | Ras-related protein Rab-3D, also known as GOV, is a member of the Ras superfamily of small Rab GTPases. Rab proteins play an important role for, either in endocytosis or in biosynthetic protein transport. Rab3D mRNA is predominantly expressed in white adipose tissue and, coincident with GLUT4, increases upon differentiation of 3T3-L1 broblasts into insulin-responsive adipocytes. Rab3D displays close structural similarities with Rab 3A, an isoform which has been functionally implicated in regulated exocytosis in various cell types. Recombinant human Rab3D protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Ras-related protein Rab-3D, GOV, RAB16 |
| Host | E. coli |
| Molecular Weight | 21.2kDa (189aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MASAGDTQAG PRDAADQNFD YMFKLLLIGN SSVGKTSFLF RYADDSFTPA FVSTVGIDFK VKTVYRHDKR IKLQIWDTAG QERYRTITTA YYRGAMGFLL MYDIANQESF AAVQDWATQI KTYSWDNAQV ILVGNKCDLE DERVVPAEDG RRLADDLGFE FFEASAKENI NVKQVFERLV DVICEKMNES LEPSSSSGSN GKGPAVGDAP APQPSSCSC |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl |
| Concentration | 0.25mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Zahraoui A., et al. (1989) J Biol Chem. 264:12934-12401. Baldini G., et al. (1992) Proc Natl Acad Sci. 89:5049-5052. |
| Background | Ras-related protein Rab-3D, also known as GOV, is a member of the Ras superfamily of small Rab GTPases. Rab proteins play an important role for, either in endocytosis or in biosynthetic protein transport. Rab3D mRNA is predominantly expressed in white adipose tissue and, coincident with GLUT4, increases upon differentiation of 3T3-L1 broblasts into insulin-responsive adipocytes. Rab3D displays close structural similarities with Rab 3A, an isoform which has been functionally implicated in regulated exocytosis in various cell types. Recombinant human Rab3D protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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