Rat 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1 (PLCZ1) ELISA Kit
| Product Name | Rat 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1 (PLCZ1) ELISA Kit |
|---|---|
| Description | Rat 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1 (PLCZ1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PLCZ1. No significant cross-reactivity or interference between Rat PLCZ1 and analogues was observed. PLCz1 Expressed specifically in testis. Weakly expressed in pancreatic-duct cells. Up-regulated in pancreatic-duct cells from patients with cystic fibrosis.The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. In vitro, hydrolyzes PtdIns(4,5)P2 in a Ca2+-dependent manner. Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage. Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion. This Rat 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase zeta-1 (PLCZ1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLCZ1 in samples. An antibody specific for PLCZ1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLCZ1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLCZ1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLCZ1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PLCZ1, MGC149685, NYD-SP27, PLCzeta, PI-phospholipase C zeta 1, testis-development related NYD-SP27 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Rat |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 497197 |
| Storage | 2-8°C |
| Supplier | Abbkine |
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