| Product Name | Rat Palmitoyl-protein thioesterase 1 (PPT1) ELISA Kit |
|---|---|
| Description | Rat Palmitoyl-protein thioesterase 1 (PPT1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PPT1. No significant cross-reactivity or interference between Rat PPT1 and analogues was observed. PPT1 is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene. The cDNA predicted a 306-amino acid polypeptide that contains a 25-amino acid signal peptide, 3 N-linked glycosylation sites, and consensus motifs characteristic of thioesterases. Northern blot analysis revealed ubiquitous expression of a single 2.5-kb mRNA, with highest expression in lung, brain, and heart. This Rat Palmitoyl-protein thioesterase 1 (PPT1) ELISA Kit employs a two-site sandwich ELISA to quantitate PPT1 in samples. An antibody specific for PPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PPT1, RP11-115D7.2, CLN1, INCL, PPT, ceroid-palmitoyl-palmitoyl-protein thioesterase 1, palmitoyl-protein hydrolase 1 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Rat |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 29411 |
| Storage | 2-8°C |
| Background |
PPT1 is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene. The cDNA predicted a 306-amino acid polypeptide that contains a 25-amino acid signal peptide, 3 N-linked glycosylation sites, and consensus motifs characteristic of thioesterases. Northern blot analysis revealed ubiquitous expression of a single 2.5-kb mRNA, with highest expression in lung, brain, and heart. |
| Supplier | Abbkine |
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