Recombinant Escherichia coli Glutamate decarboxylase alpha (gadA)
| Product Name | Recombinant Escherichia coli Glutamate decarboxylase alpha (gadA) |
|---|---|
| Description | Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad syst helps to maintain a near-neutral intracellular pH when cells are exposed to extrely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria. |
| Host | E.coli |
| Molecular Weight | 56.7 |
| Amino Acid Sequence | MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT |
| Protein Length | Full Length, 1-466aa |
| Tag | N-terminal 6xHis-tagged |
| Reactivity | E.coli |
| Applications | SDS-PAGE |
| Form | Liquid, in Tris-based buffer, 50% glycerol |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| References | Transcriptional expression of Escherichia coli glutamate-dependent acid resistance genes gadA and gadBC in an hns rpoS mutant.Waterman S.R., Small P.L.C.J. Bacteriol. 185:4644-4647(2003) |
| Background | Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad syst helps to maintain a near-neutral intracellular pH when cells are exposed to extrely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria. |
| Supplier | Cusabio |
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