| Product Name | RETN ELISA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Resistin (RETN). No significant cross-reactivity or interference between Resistin (RETN) and analogues was observed. |
| Synonyms | ADSF; FIZZ3; RETN1; RSTN; XCP1; Adipose tissue-specific secretory factor; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted FIZZ3 |
| Method | Sandwich |
| Detection Range | 0.156-10ng/mL |
| Sensitivity | 0.065ng/mL |
| Target | RETN |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Resistin (RETN). No significant cross-reactivity or interference between Resistin (RETN) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | Q8K4J7 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat diet;Tinospora crispa Ameliorates Insulin Resistance Induced by High Fat Diet in Wistar Rats;Estradiol stimulates mitochondrial biogenesis and adiponectin expression in skeletal muscle;Adipose tissue regulates hepatic cholesterol metabolism via adiponectin;Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats;Tinospora crispa;Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokines;Four-Week Consumption of Malaysian Honey Reduces Excess Weight Gainand Improves Obesity-Related Parameters in High Fat Diet Induced ObeseRats.;Biological effects of adipocytes in sulfur mustard induced toxicity;Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal rats;Mango leaf tea promotes hepatoprotective effects in obese rats;Bacupari (Garcinia brasiliensis) extract modulates intestinal microbiota and reduces oxidative stress and inflammation in obese rats;Extruded sorghum (Sorghum bicolor L.) improves gut microbiota, reduces inflammation, and oxidative stress in obese rats fed a high-fat diet;Antidiabetic and cardiovascular beneficial effects of a liver-localized mitochondrial uncoupler; |
| Supplier | Cloud Clone |
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