| Product Name | REV1, 51-256aa, Human |
|---|---|
| Description | REV1 contains a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found. Recombinant human REV1 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | DNA repair protein REV1, REV1L |
| Host | E. coli |
| Molecular Weight | 33.9kDa (296aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGVAIYVNGY TDPSAEELRK LMMLHGGQYH VYYSRSKTTH IIATNLPNAK IKELKGEKVI RPEWIVESIK AGRLLSYIPY QLYTKQSSVQ KGLSFNPVCR PEDPLPGPSN IAKQLNNRVN HIVKKIETEN EVKVNGMNSW NEEDENNDFS FVDLEQTSPG RKQNGIPHPR GSTAIFNGHT PSSNGALKTQ DCLVPMVNSV ASRLSPA |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol. |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Gibbs P.E.M., et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97:4186-4191 Masuda Y., et al. (2001) J. Biol. Chem. 276:15051-15058 |
| Background | REV1 contains a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found. Recombinant human REV1 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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