| Product Name | RNASE7, 29-156aa, Human His tag |
|---|---|
| Description | RNASE7 is one of the final RNase A superfamily ribonucleases. It was isolated from skin-derived stratum corneum. This protein exhibited potent ribonuclease activity and thus may contribute to the well known ribonuclease activity of human skin. It revealed broad spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity against a vancomycin-resistant Enterococcus faecium. Recombinant human RNASE7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Synonyms | Ribonuclease, RNase A family, 7, MGC133220, Ribonuclease RNase A family 7, RNase 7, Ribonuclease 7, Skin-derived antimicrobial protein 2 |
| Host | E. coli |
| Molecular Weight | 27.1 kDa (239aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSKPKGMTS SQWFKIQHMQ PSPQACNSAM KNINKHTKRC KDLNTFLHEP FSSVAATCQT PKIACKNGDK NCHQSHGPVS LTMCKLTSGK YPNCRYKEKR QNKSYVVACK PPQKKDSQQF HLVPVHLDRV L |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Zhang J. et al. (2003) Nucleic Acids Res. 31:602-607 Harder J. et al. (2002) J Biol Chem. 277:46779-46784. |
| Background | RNASE7 is one of the final RNase A superfamily ribonucleases. It was isolated from skin-derived stratum corneum. This protein exhibited potent ribonuclease activity and thus may contribute to the well known ribonuclease activity of human skin. It revealed broad spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity against a vancomycin-resistant Enterococcus faecium. Recombinant human RNASE7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. |
| Supplier | ARP |
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