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|Product Name||RYBP, 1-228aa, Human|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MGSHMTMGDK KSPTRPKRQA KPAADEGFWD CSVCTFRNSA EAFKCSICDV RKGTSTRKPR INSQLVAQQV AQQYATPPPP KKEKKEKVEK QDKEKPEKDK EISPSVTKKN TNKKTKPKSD ILKDPPSEAN SIQSANATTK TSETNHTSRP RLKNVDRSTA QQLAVTVGNV TVIITDFKEK TRSSSTSSST VTSSAGSEQQ NQSSSGSEST DKGSSRSSTP KGDMSAVNDE SF|
|Background||RYBP (RING1- and YY1-binding protein) is a member of the polycomb group (PcG). This protein interacts with MDM2 and decreases MDM2-mediated p53 ubiquitination, leading to stabilization of p53 and an increase in p53 activity. RYBP induces cell-cycle arrest and is involved in the p53 response to DNA damage. RYBP has been shown to interact with RING1, YY1, Caspase 10, E2F3, E2F2, Mdm2, Abl gene and CBX2. Recombinant human RYBP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||0.25mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20 mM Tris-HCl buffer, pH8.0, 40% glycerol, 5mM DTT, 200mM NaCl|
|Intended Use||For Research Use Only|
|Molecular Weight||25.2kDa (225aa) confirmed by MALDI-TOF|
|NCBI Accession #||NP_036366|
|Purity||> 85% by SDS-PAGE|
|References||Chen D, et al. (2008) EMBO Rep. 10(2):166-72.
Zheng L., et al. (2001) J. Biol. Chem. 276:31945-31952
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||RING1 and YY1 binding protein, AAP1, DEDAF, YEAF1|
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