| Product Name | S100A3, 1-101aa Mouse |
|---|---|
| Description | S100A3, also known as Protein S-100E and S100 calcium-binding protein A3, belongs to the S-100 family of proteins containing 2 EF-hand calcium-binding motifs. The S100 proteins act as multifactional signaling factors that are involved in the regulation of diverse cellular processes such as cell cycle progression and differentiation. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. This protein has the highest content of cysteines of all S100 proteins, has a high affinity for Zinc, and is highly expressed in human hair cuticle. Recombinant mouse S100A3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | S100 calcium binding protein A3, S100E |
| Host | E. coli |
| Molecular Weight | 42 kDa (376aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMTRPLE QAVAAIVCTF QEYAGRCGDK YKICQSELKE LLQKELPTWT PSEFRECDYN KFMSVLDTNK DCEVDFGEYV RSLASLCLYC HEYFKECPPE PPCPQ |
| Tag | His-tag |
| Reactivity | Mouse |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Streicher WW, et al. (2010) Biophys Chem. 151(3):181-6. Yao R, et al. (2007) Anticancer Res. 27(5A):3051-8. |
| Background | S100A3, also known as Protein S-100E and S100 calcium-binding protein A3, belongs to the S-100 family of proteins containing 2 EF-hand calcium-binding motifs. The S100 proteins act as multifactional signaling factors that are involved in the regulation of diverse cellular processes such as cell cycle progression and differentiation. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. This protein has the highest content of cysteines of all S100 proteins, has a high affinity for Zinc, and is highly expressed in human hair cuticle. Recombinant mouse S100A3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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